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Characterization of fungal community in spruce (Picea abies) litter using cultivation and T-RFLP
Kolářová, Zuzana ; Koukol, Ondřej (advisor) ; Brabcová, Vendula (referee)
Fungi have a key role in the decomposition of coniferous litter and affect nutrient cycling in forest ecosystems. Therefore, great emphasis is placed on exploring the diversity of these organisms. The aim of this thesis was to describe fungal diversity in spruce litter and revealed temporal development of this community in a forest regenerating after bark beetle outbreak. Another objective was to compare sites with different length of bark beetle damage. The study area was located in the Bohemian Forest mountain range. Litter bags with spruce needles were placed on the forest soil and several samplings were performed in the course of three years. Diversity of fungi and changes in the fungal community were assessed by two methods: cultivation of needles on 2ř MEA and fingerprinting method T-RFLP. In total 71 fungal species were obtained from needles during a three-year succession by cultivation approach. Using T-RFLP 122 different fragments were generally recorded. The dominant species were Scleroconidioma sphagnicola, Thysanophora penicillioides, Hormonema dematiodes, Ceuthospora pinastri, species of genus Chalara, Trichoderma polysporum, Mycena galopus and unknown species Helotiales sp. 1. Primary saprotrophs occured in the community mainly in first 8 months and then were replaced by basidiomycetes....
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Characterization of fungal community in spruce (Picea abies) litter using cultivation and T-RFLP
Kolářová, Zuzana ; Koukol, Ondřej (advisor) ; Brabcová, Vendula (referee)
Fungi have a key role in the decomposition of coniferous litter and affect nutrient cycling in forest ecosystems. Therefore, great emphasis is placed on exploring the diversity of these organisms. The aim of this thesis was to describe fungal diversity in spruce litter and revealed temporal development of this community in a forest regenerating after bark beetle outbreak. Another objective was to compare sites with different length of bark beetle damage. The study area was located in the Bohemian Forest mountain range. Litter bags with spruce needles were placed on the forest soil and several samplings were performed in the course of three years. Diversity of fungi and changes in the fungal community were assessed by two methods: cultivation of needles on 2ř MEA and fingerprinting method T-RFLP. In total 71 fungal species were obtained from needles during a three-year succession by cultivation approach. Using T-RFLP 122 different fragments were generally recorded. The dominant species were Scleroconidioma sphagnicola, Thysanophora penicillioides, Hormonema dematiodes, Ceuthospora pinastri, species of genus Chalara, Trichoderma polysporum, Mycena galopus and unknown species Helotiales sp. 1. Primary saprotrophs occured in the community mainly in first 8 months and then were replaced by basidiomycetes....
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The role of wood decay fungi in the dynamics of a mountain spruce forest
POUSKA, Václav
This thesis is focused on environmental preferences of wood-decaying fungi and their relationships with forest structure and development. Relationships of fungi to properties of wood and forest stands were studied on the basis of field observations in Central-European mountain spruce forests. Plot-based approach was used to reveal a general pattern in the diversity of fungi within a single forest stand and between different stands. The analysis of stand structure provided a background for plot-based approach. Substrate-based approach was used to study single species preferences and their communities. In addition, the influence of wood properties (including fungi and their rots) on the regeneration of spruce on logs was studied.
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